PRP Kits
As mentioned above, most pharmacies stock PRP kits. Within these kits is everything needed for phlebotomy and the tubes needed for collecting and spinning the blood, as well as needles to extract the platelets. These kits range from around £55-£65 plus VAT. We use PRPHD.
| Diagram D – PRPHD Kit
PRP Kit Contents |
| Blood collection needle & holder |
| 2x PRP vacuumed blood collecting tubes (10ml each) |
| 1 & 5ml syringe |
| Plasma tube (5ml) |
| Needles for plasma extraction |
| Needles for Injection (30g) |
Diagram E – Technique of PRP kit in action
Above is a diagram of how to extract blood using the PRP Kits.
- Draw blood from the patient, using blood holder and butterfly needle, and fill the PRP tube with whole blood.
- Up and down the PRP tubes 3 times, to make the blood with anticoagulant.
- Place the PRP tube into centrifuge. Always ensure the centrifuge is correctly balanced before start. Please centrifuge at 1800 (RCF) for 10 minutes.
4. After centrifugation, the RBC and most of WBC will be below the plug of gel.
Gently in vert the PRP tube several times to re-suspend the platelets in the plasma.
5. Mixing the plasma supernatant and Platelet Rich Plasma for 5 times.
6. Put 18G spinal needle on syringe and inject into centrifuged PRP tube to extract platelet rich plasma.